An Investigation to Show the Effect of Temperature on Daphnia :: Papers

An Investigation to raise the Effect of Temperature on Daphnia Plan I will go at a lower place some daphnia contained in a test tube in water of differing temperatures and measure their subsequent shopping centre rates. Hypothesis I look that the heart rate of the daphnia will increase up until rough 40C at which point most of the daphnias enzymes will film denatured and rate of metabolism will have stopped or decreased sufficiently to have stopped the daphnias heart rate. Apparatus tilt Test tube Beaker Different temperatures of water Daphnia Pipette ascertain clock Microscope Slide Safety Follow usual lab sentry go rules. There argon no major safety considerations in this examine as the daphnia arent harmful and water above 40C will not be used. Method To determine if temperature does have an depression on the daphnia, I intend to carry out the following experiment. The experiment will involve measuring the cardiac acti vity of the daphnia at different temperatures, ranging from very cold (approx. 5 degrees Celsius) to quite warm (approx. 40 degrees Celsius). A selection of similar sized/age Daphnia will be taken. A variety of different temperatures of water will be set up, these temperatures will be kept constant whilst the daphnia are submersed in them. The temperatures will be set up by using ice to cool tap water down to degrade temperatures and boiling water to heat it up. The temperature will be measured to inside 0.1C on a mercury thermometer to ensure accuracy. The temperatures will ladder from 10C to approximately 40C and it will be done at 5C intervals. The daphnia will be submersed in the water and left to equilibrate for 3 minutes, afterward this time one of the Daphnia will be removed and lay onto a microscope slide, this will then be quickly put under the microscope. -1- Under the microscope, the Daphnia will be observed for 20 seconds, this h eart rate will be counted and recorded, this can the be